Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig5: Flow cytometric analysis of CD34 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-01, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CD34 [15H1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Both isoforms are expressed on the cell surface. CD34-T/CD34-F ratio increases with cell differentiation,developmental stage:On early hematopoietic progenitor cells.,disease:Abnormal CD34 expression in leukemogenesis.,function:Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.,online information:CD34 entry,PTM:Highly glycosylated.,PTM:Phosphorylated on serine residues by PKC.,similarity:Belongs to the CD34 family.,tissue specificity:Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues., |
| Immunogen | Recombinant protein within N-terminal Rat CD34. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHC-P,FC |
| IHC-P:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 2mg/mL |
| Purification | Unpurified |
| Alternative Names | CD34 antibody
CD34 antigen antibody
CD34 molecule antibody CD34_HUMAN antibody Cluster designation 34 antibody Hematopoietic progenitor cell antigen CD34 antibody HPCA1 antibody Mucosialin antibody OTTHUMP00000034733 antibody OTTHUMP00000034734 antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa (Predicted band size) |
| Cellular Localization | Plasma membrane. |
Application
Fig1: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-01, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Flow cytometric analysis of CD34 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-01, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Rat brain tissue, rat kidney tissue, human liver carcinoma tissue, mouse kidney tissue, THP-1. |
|---|---|
| Application Notes | IHC-P:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
