Application
Fig1: Western blot analysis of NM23 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysateApplication
Fig2: Western blot analysis of NM23 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig3: Flow cytometric analysis of NM23 was done on293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-18, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | NM23 [13C2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. |
| Immunogen | Synthetic peptide within Human NM23 aa 10-30. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,ICC,FC |
| WB:1:2,000-1:5,000 ICC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | AWD antibody
AWD drosophila homolog of antibody GAAD antibody Granzyme A activated DNase antibody Granzyme A-activated DNase antibody GZMA activated DNase antibody Metastasis inhibition factor NM23 antibody NB antibody NBS antibody NDK A antibody NDKA antibody NDKA_HUMAN antibody NDP kinase A antibody NDPK-A antibody NDPKA antibody NM23 antibody NM23 long variant included antibody nm23-H1 antibody NM23-M1 antibody NM23H1B included antibody NME/NM23 nucleoside diphosphate kinase 1 antibody Nme1 antibody NME1-NME2 spliced read-through transcript included antibody Non-metastatic cells 1 protein (NM23A) expressed in antibody Nonmetastatic cells 1 protein expressed in antibody Nonmetastatic protein 23 antibody Nonmetastatic protein 23 homolog 1 antibody Nucleoside diphosphate kinase A antibody Tumor metastatic process-associated protein antibody |
|---|---|
| Molecular Weight(MW) | 17/19 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P15531 |
|---|
Application
Fig1: Western blot analysis of NM23 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate
Application
Fig2: Western blot analysis of NM23 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig3: Flow cytometric analysis of NM23 was done on293 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-18, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | HepG2 cell, A549 cell, K562 cell, 293cell. |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
