Application
Fig1: Western blot analysis of Thymidine Phosphorylase on A549 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-23, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig5: Flow cytometric analysis of Thymidine Phosphorylase was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-23, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Thymidine Phosphorylase [A1A8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Platelet-derived endothelial cell growth factor (PD-ECGF), which is alternatively designated thymidine phosphorylase or gliostatin, is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Biologically active PD-ECGF is a functional dimer that consists of two single polypeptide chains that are expressed in platelets, placenta, foreskin fibroblasts and various squamous cell carcinomas, and they are slowly secreted from the cells. In addition, PD-ECGF is overexpressed in tumor and lesional psoriatic skin and lesional epidermis, indicating that it may play a role in the pathophysiology of psoriasis. Serine residues of PD-ECGF are frequently associated with nucleotide triphosphates, including ATP. In an ATP dependent manner, PD-ECGF is also able to catalyze the reversible phosphorolysis of thymidine to thymine, as it contains thymidine phosphorylase activities. |
| Immunogen | Recombinant protein within human Thymidine Phosphorylase aa 1-200. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB,ICC,IHC,FC |
| WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/mL |
| Purification | Unpurified |
| Alternative Names | ECGF 1 antibody
ECGF antibody
ECGF1 antibody Endothelial cell growth factor 1 antibody Endothelial cell growth factor 1 platelet derived antibody Endothelial cell growth factor platelet-derived antibody Gliostatin antibody hPD ECGF antibody MEDPS1 antibody MNGIE antibody MTDPS1 antibody PD ECGF antibody PD-ECGF antibody PDECGF antibody PDEGF antibody Platelet derived endothelial cell growth factor antibody Platelet derived endothelial growth factor antibody Platelet-derived endothelial cell growth factor antibody TdRPase antibody Thymidine phosphorylase antibody TP antibody Tymp antibody TYPH_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytosol, nuclear. |
| SwissProt ID | P19971 |
|---|
Application
Fig1: Western blot analysis of Thymidine Phosphorylase on A549 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-23, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Thymidine Phosphorylase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-23, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig5: Flow cytometric analysis of Thymidine Phosphorylase was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-23, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | A549 cell, human liver carcinoma tissue, human appendix tissue, human spleen tissue, SiHa cell. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
