Application
Fig1: Western blot analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Application
Fig3: Flow cytometric analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | ZAP70 [A1B5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 70 kDa zeta associated protein antibody 70 kDa zeta-associated protein antibody EC 2.7.10.2 antibody FLJ17670 antibody FLJ17679 antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Syk-related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody Tyrosine-protein kinase ZAP-70 antibody TZK antibody ZAP 70 antibody ZAP70 antibody ZAP70_HUMAN antibody Zeta chain associated protein kinase 70kD antibody Zeta chain associated protein kinase 70kDa antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody Zeta chain of T cell receptor associated protein kinase 70 antibody Zeta chain TCR associated protein kinase 70kD antibody Zeta chain TCR associated protein kinase 70kDa antibody |
| Product description | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. |
| Immunogen | Recombinant protein within human ZAP70 aa 250-480. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBIHC-PFCICC |
| WB:1:500-1:2,000 IHC-P:1:50-1:100 FC:1:50-1:100 ICC:1:50 |
|
| Species Reactivity | Human |
| Concentration | 2mg/mL |
| Alternative Names | 70 kDa zeta associated protein antibody
70 kDa zeta-associated protein antibody
EC 2.7.10.2 antibody FLJ17670 antibody FLJ17679 antibody Selective T cell defect antibody SRK antibody STD antibody Syk related tyrosine kinase antibody Syk-related tyrosine kinase antibody Truncated ZAP kinase antibody Tyrosine protein kinase ZAP70 antibody Tyrosine-protein kinase ZAP-70 antibody TZK antibody ZAP 70 antibody ZAP70 antibody ZAP70_HUMAN antibody Zeta chain associated protein kinase 70kD antibody Zeta chain associated protein kinase 70kDa antibody Zeta chain associated protein kinase 70kDa isoform 1 antibody Zeta chain associated protein kinase 70kDa isoform 2 antibody Zeta chain of T cell receptor associated protein kinase 70 antibody Zeta chain TCR associated protein kinase 70kD antibody Zeta chain TCR associated protein kinase 70kDa antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cell membrane, cytoplasm. |
| SwissProt ID | P43403 |
|---|
Application
Fig1: Western blot analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1901-27, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application
Fig3: Flow cytometric analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Jurkat cell, human tonsil tissue, human colon tissue, human spleen tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC-P:1:50-1:100 FC:1:50-1:100 ICC:1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
