Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | SPATIAL [16A4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ12286 antibody MGC5347 antibody SPA5L_HUMAN antibody SPATA5L1 antibody Spermatogenesis-associated protein 5-like protein 1 antibody |
| Product description | To date, there have been several linkage studies to identify genetic loci determining renal function in individuals with renal disease or in the normal population. Puppala et al. suggested candidate regions including 2q36.3 and 9q21.31–q21.33 in the Mexican–American population, and Schelling et al. reported the 1q43, 7q36.1, 8q13.3, and 18q23.3 regions using multiethnic diabetic populations. There are several additional regions that have been suggested from other linkage studies, including 7p15.3–p13, 12p12.2, and 16q12.2–16q23.1. In recent years, researchers have tried to determine in more detail the genetic basis of the estimated GFR (eGFR) through genome-wide association studies (GWAS), and have begun suggesting some genes or variants as determinants of eGFR or chronic kidney disease. These studies, based on a large number of samples, have identified several variants showing a high level of significance and reproducibility near or within genes including UMOD, SHROOM3, GATM, and SPATA5L1. |
| Immunogen | Synthetic peptide within human aa 350-600. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/mL |
| Alternative Names | FLJ12286 antibody
MGC5347 antibody
SPA5L_HUMAN antibody SPATA5L1 antibody Spermatogenesis-associated protein 5-like protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 80/41 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q9BVQ7 |
|---|
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig8: Flow cytometric analysis of SPATIAL was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | K562 cell lysates, rat seminal vesicle tissue, human skin tissue, human breast carcinoma tissue, human placenta tissue, human small intestine tissue, mouse kidney tissue, JAR. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
