Application
Fig1: Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysateApplication
Fig2: ICC staining of Aspartate Aminotransferase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: Flow cytometric analysis of Aspartate Aminotransferase was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-51, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Aspartate Aminotransferase [A2D6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. |
| Immunogen | Recombinant protein within human GOT1 aa 10-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WB,ICC,FC |
| WB:1:500-1:2,000 ICC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Purification | Unpurified |
| Alternative Names | AATC_HUMAN antibody
Aspartate aminotransferase 1 antibody
Aspartate aminotransferase antibody Aspartate aminotransferase cytoplasmic antibody Aspartate aminotransferase cytosolic antibody ASTQTL1 antibody cAspAT antibody cCAT antibody cysteine aminotransferase cytoplasmic antibody cysteine transaminase cytoplasmic antibody cytoplasmic antibody ec 2.6.1.1 antibody GIG 18 antibody GIG18 antibody Glutamate oxaloacetate transaminase 1 antibody Glutamate oxaloacetate transaminase soluble antibody Glutamic oxaloacetic transaminase 1 soluble antibody GOT 1 antibody GOT1 antibody Growth inhibiting protein 18 antibody SGOT antibody Transaminase A antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P17174 |
|---|
Application
Fig1: Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: K562 cell lysate
Application
Fig2: ICC staining of Aspartate Aminotransferase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: Flow cytometric analysis of Aspartate Aminotransferase was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-51, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | HepG2 cell lysate, K562 cell lysate, SiHa, SW620. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
