Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | UBA3 [1B6-6-5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DKFZp566J164 antibody EC 6.3.2. antibody hUba3 antibody MGC22384 antibody NEDD8 activating enzyme E1 catalytic subunit antibody NEDD8 activating enzyme E1C antibody Nedd8 activating enzyme hUba3 antibody NEDD8-activating enzyme E1 catalytic subunit antibody NEDD8-activating enzyme E1C antibody uba3 antibody UBA3 ubiquitin activating enzyme E1 homolog antibody UBA3_HUMAN antibody UBE1C antibody Ubiquitin activating enzyme 3 antibody Ubiquitin activating enzyme E1C antibody Ubiquitin-activating enzyme 3 antibody Ubiquitin-activating enzyme E1C antibody Ubiquitin-like modifier-activating enzyme 3 antibody |
| Product description | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Immunogen | Recombinant protein within human UBA3 aa 200-463. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC-PFC |
| WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | DKFZp566J164 antibody
EC 6.3.2. antibody
hUba3 antibody MGC22384 antibody NEDD8 activating enzyme E1 catalytic subunit antibody NEDD8 activating enzyme E1C antibody Nedd8 activating enzyme hUba3 antibody NEDD8-activating enzyme E1 catalytic subunit antibody NEDD8-activating enzyme E1C antibody uba3 antibody UBA3 ubiquitin activating enzyme E1 homolog antibody UBA3_HUMAN antibody UBE1C antibody Ubiquitin activating enzyme 3 antibody Ubiquitin activating enzyme E1C antibody Ubiquitin-activating enzyme 3 antibody Ubiquitin-activating enzyme E1C antibody Ubiquitin-like modifier-activating enzyme 3 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytosol, nucleus, cytoplasm. |
| SwissProt ID | Q8TBC4 |
|---|
Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of UBA3 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | 293T cell lysates, human lung tissue, human lung carcinoma tissue, human breast carcinoma tissue, SiHa. |
|---|---|
| Application Notes | WB:1:500-1:2,000: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
