Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CEACAM6 [A1E3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carcinoembryonic antigen related cell adhesion molecule 6 antibody Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody Carcinoembryonic antigen-related cell adhesion molecule 6 antibody CD 66c antibody CD66c antibody CD66c antigen antibody CEA LIKE PROTEIN antibody CEACAM 6 antibody CEACAM6 antibody CEAL antibody CEAM6_HUMAN antibody MGC93832 antibody NCA antibody Non specific cross reacting antigen antibody Non-specific crossreacting antigen antibody Normal cross reacting antigen antibody Normal cross-reacting antigen antibody |
| Product description | Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells. Plays a role as an oncogene by promoting tumor progression; positively regulates cell migration, cell adhesion to endothelial cells and cell invasion. Also involved in the metastatic cascade process by inducing gain resistance to anoikis of pancreatic adenocarcinoma and colorectal carcinoma cells. |
| Immunogen | Recombinant protein within human CEACAM6 aa 1-150. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ICCIHC-PFC |
| ICC:1:50-1:100: IHC-P:1:50-1:200: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Carcinoembryonic antigen related cell adhesion molecule 6 antibody
Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
Carcinoembryonic antigen-related cell adhesion molecule 6 antibody CD 66c antibody CD66c antibody CD66c antigen antibody CEA LIKE PROTEIN antibody CEACAM 6 antibody CEACAM6 antibody CEAL antibody CEAM6_HUMAN antibody MGC93832 antibody NCA antibody Non specific cross reacting antigen antibody Non-specific crossreacting antigen antibody Normal cross reacting antigen antibody Normal cross-reacting antigen antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 37 kDa. |
| Cellular Localization | Cell membrane, apical cell membrane, cell surface. |
| SwissProt ID | P40199 |
|---|
Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application
Fig5: Flow cytometric analysis of CEACAM6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-69, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | HT-29, human lung tissue, human colon tissue, human colon carcinoma tissue, SW620. |
|---|---|
| Application Notes | ICC:1:50-1:100: IHC-P:1:50-1:200: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
