Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.| Product Name | beta I Tubulin [5F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2810484G07Rik Beta tubulin 1, class VI Class VI beta tubulin dJ543J19.4 M(beta)1 TBB1_HUMAN TUBB1 Tubulin beta 1 class VI Tubulin beta-1 chain Tubulin, beta 1 tubulin, beta1 |
| Product description | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human beta I Tubulin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC-PFC |
| WB:1:5000-20000: IHC-P:1:400-800: FC:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 2810484G07Rik
Beta tubulin 1 class VI Class VI beta tubulin dJ543J19.4 M(beta)1 TBB1_HUMAN TUBB1 Tubulin beta 1 class VI Tubulin beta-1 chain Tubulin beta 1 tubulin beta1 |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton |
| SwissProt ID | Q9H4B7 |
|---|
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.| Positive Control | Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. |
|---|---|
| Application Notes | WB:1:5000-20000: IHC-P:1:400-800: FC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
