Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- HSP40 (EM2001-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kDApplication
Fig2: Sample: U251(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- HSP40 (EM2001-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD| Product Name | HSP40 [3G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSP40 homolog DnaJ (Hsp40) homolog subfmaily B member 1 DNAJ 1 DNAJ B1 DnaJ homolog subfamily B member 1 DnaJ protein homolog 1 DNAJ1 DNAJB 1 Dnajb1 DNAJB1 protein DNJB1_HUMAN HDJ 1 HDJ-1 HDJ1 Heat shock 40 kDa protein 1 Heat shock 40kD protein 1 Heat shock protein 40 Hsp 40 HSP40 HSPF 1 HSPF1 Human DnaJ protein 1 Radial spoke 16 homolog B RSPH16B Sis1. |
| Product description | Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. |
| Immunogen | Recombinant human HSP40 Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HSP40 homolog DnaJ (Hsp40) homolog subfmaily B member 1 DNAJ 1 DNAJ B1 DnaJ homolog subfamily B member 1 DnaJ protein homolog 1 DNAJ1 DNAJB 1 Dnajb1 DNAJB1 protein DNJB1_HUMAN HDJ 1 HDJ-1 HDJ1 Heat shock 40 kDa protein 1 Heat shock 40kD protein 1 Heat shock protein 40 Hsp 40 HSP40 HSPF 1 HSPF1 Human DnaJ protein 1 Radial spoke 16 homolog B RSPH16B Sis1. |
|---|---|
| Molecular Weight(MW) | 38 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. |
| SwissProt ID | P25685 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti- HSP40 (EM2001-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD
Application
Fig2: Sample: U251(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- HSP40 (EM2001-05) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD| Positive Control | Expressed in heart, pancreas and skeletal muscle, and to a lesser extent in brain, placenta and liver. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
