Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- AMPK gamma1 (EM2001-06) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Product Name | AMPK gamma1 [5A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKAG 1 PRKAG1 5 -AMP-activated protein kinase gamma-1 subunit 5' AMP activated protein kinase subunit gamma 1 AMP activated protein kinase noncatalytic gamma 1 subunit AMPK gamma 1 chain AMPKg MGC8666 PRKAG 1 PRKAG1 Protein kinase AMP-activated gamma 1 non-catalytic subunit. |
| Product description | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Immunogen | Recombinant human AMPK gamma1 Protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | PRKAG 1 PRKAG1 5 -AMP-activated protein kinase gamma-1 subunit 5' AMP activated protein kinase subunit gamma 1 AMP activated protein kinase noncatalytic gamma 1 subunit AMPK gamma 1 chain AMPKg MGC8666 PRKAG 1 PRKAG1 Protein kinase AMP-activated gamma 1 non-catalytic subunit. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Cytosol; Nucleus |
| SwissProt ID | P54619 |
|---|
Application
Fig1: Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- AMPK gamma1 (EM2001-06) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
