WB
Western blot analysis of Myc-tag protein on different cell lysates using anti-Myc-tag antibody at 1/2000 dilution. Positive control: Lane 1: N-terminal Myc-tag protein, Lane 2: C-terminal Myc-tag protein.ICC/IF
ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | Myc tag Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This Myc-Tag antibody detects Myc-tagged fusion proteins. |
| Immunogen | Synthetic peptide immune sequence is EQKLISEEDLC-KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| WB:1:2000 ICC/IF:1:50 IP:2-5 µg/ml |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Alternative Names | avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c-Myc antibody class E basic helix-loop-helix protein 39 antibody MRTL antibody MYC antibody Myc Epitope Tag antibody myc proto-oncogene protein antibody myc-related translation/localization regulatory factor antibody oncogene c-Myc antibody proto-oncogene c-Myc antibody protooncogene homologous to myelocytomatosis virus antibody transcription factor p64 antibody v-myc avian myelocytomatosis viral oncogene homolog antibody v-myc myelocytomatosis viral oncogene homolog (avian) antibody |
|---|
WB
Western blot analysis of Myc-tag protein on different cell lysates using anti-Myc-tag antibody at 1/2000 dilution. Positive control: Lane 1: N-terminal Myc-tag protein, Lane 2: C-terminal Myc-tag protein.
ICC/IF
ICC staining of Myc tag in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:2000 ICC/IF:1:50 IP:2-5 µg/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
