IHC
Species: Human, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody:1/1,000, 1 hour at room temperature. Secondary antibody:20 minutes at room temperature.IHC
Species: Rat, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody:1/1,000, 1 hour at room temperature. Secondary antibody:20 minutes at room temperature.IF-P
Species: Human, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody:1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-P
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-P
Species: Rat, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Product Name | Cytokeratin 10 Recombinant Antibody- Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum. |
| Immunogen | Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584. |
| Clonality | Monoclonal |
|---|---|
| Tested Applications | IF-PIHC |
| IHC:1:1000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | BCIE antibody BIE antibody CK 10 antibody CK-10 antibody Cytokeratin-10 antibody EHK antibody K10 antibody K1C10_HUMAN antibody Keratin 10 antibody Keratin antibody Keratin type i cytoskeletal 10 antibody Keratin type I cytoskeletal 59 kDa antibody Keratin-10 antibody Keratin10 antibody KPP antibody KRT10 antibody type I cytoskeletal 10 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa |
| Cellular Localization | Secreted, extracellular space, Cell surface, Cytoplasm. |
IHC
Species: Human, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody:1/1,000, 1 hour at room temperature. Secondary antibody:20 minutes at room temperature.
IHC
Species: Rat, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody:1/1,000, 1 hour at room temperature. Secondary antibody:20 minutes at room temperature.
IF-P
Species: Human, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody:1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-P
Species: Mouse, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-P
Species: Rat, Tissue: Skin, Sample: Paraffin-embedded section, Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST, Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Application Notes | IHC:1:1000 IF-P:1:1000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
