WB
Western blot analysis of AGTR2 on different lysates using anti-AGTR2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: HelaWB
Western blot analysis of Angiotensin II Type 2 Receptor on different lysates with Rabbit anti-Angiotensin II Type 2 Receptor antibody at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Rat liver tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 30 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Angiotensin II Type 2 Receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Angiotensin II (Ang II) is an important physiological effector of blood pressure and volume regulation through vasoconstriction, aldosterone release, sodium uptake and thirst stimulation. Although Ang II interacts with two types of cell surface receptors, AT1 and AT2, most of the major cardiovascular effects seem to be mediated through AT1. Molecular cloning of the AT1 protein has shown it to be a member of the G protein-associated seven transmembrane protein receptor family. Ang II treatment of cells results in activation of several signal transduction pathways as evidenced by tyrosine phosphorylation of several proteins and induction of others. PLCγ is phosphorylated after 30 seconds of treatment with Angiotensin II, indicating this as an early signal transduction event. Ang II treatment also stimulates phosphorylation of Shc, FAK and MAP kinases, and induces MKP-1, indicating stimulation of growth factor pathways. Ang II stimulation through AT1 has been shown to activate the JAK/Stat pathway involving a direct interaction between JAK2 and AT1 as demonstrated by coimmunoprecipitation. The AT1 receptor has no cytoplasmic kinase domain, but is able to function as a substrate for Src kinases and has several putative phosphorylation sites. |
| Immunogen | Synthetic peptide within Human Angiotensin II Type 2 Receptoraa 311-363 / 363. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AGTR2 |
|---|---|
| Gene Synonyms | AT2 ATGR2 MRX88 |
| Gene Full Name | angiotensin II receptor type 2 |
| Alternative Names | AGTR 2 antibody Agtr2 antibody AGTR2_HUMAN antibody angiotensin II receptor type 2 antibody Angiotensin II type-2 receptor antibody Angiotensin receptor 2 antibody AT 2 antibody AT2 antibody ATGR 2 antibody ATGR2 antibody MRX 88 antibody MRX88 antibody Type 2 angiotensin II receptor antibody Type-2 angiotensin II receptor antibody |
| Molecular Weight(MW) | 41kDa(Observed band size: 45kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of AGTR2 on different lysates using anti-AGTR2 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela
WB
Western blot analysis of Angiotensin II Type 2 Receptor on different lysates with Rabbit anti-Angiotensin II Type 2 Receptor antibody at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate, Lane 2: Mouse liver tissue lysate, Lane 3: Rat liver tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 30 seconds; Lane 3: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Positive Control | 293T, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
