Application
Fig1: Western blot analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).Application
Fig2：Immunofluorescent staining of Hela cells using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).Application
Fig3：Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).Application
Fig4: Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution.Application
Fig5: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin.| Product Name | 14-3-3 b/a [9-26] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 14 3 3 alpha antibody 14 3 3 protein beta/alpha antibody 14-3-3 protein beta/alpha antibody 1433B_HUMAN antibody Brain protein 14 3 3 beta isoform antibody GW128 antibody HS 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein 1054 antibody Protein kinase C inhibitor protein 1 antibody YWHAA antibody YWHAB antibody |
| Product description | The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints. The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins. Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol. The 14-3-3 proteins are also Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. They bind to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:1,000-1:2,000 ICC:1:100 IHC:1:200 |
|
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 2mg/ml |
| Alternative Names | 14 3 3 alpha antibody
14 3 3 protein beta/alpha antibody 14-3-3 protein beta/alpha antibody 1433B_HUMAN antibody Brain protein 14 3 3 beta isoform antibody GW128 antibody HS 1 antibody KCIP-1 antibody KCIP1 antibody N-terminally processed antibody Protein 1054 antibody Protein kinase C inhibitor protein 1 antibody YWHAA antibody YWHAB antibody |
|---|---|
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P31946 |
|---|
Application
Fig1: Western blot analysis on mouse liver lysates using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
Application
Fig2：Immunofluorescent staining of Hela cells using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
Application
Fig3：Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using anti-14-3-3b/a Mouse mAb (Cat. # M0407-14).
Application
Fig4: Western blot analysis of 14-3-3b/a on hybrid fish (crucian-carp) brain tissue lysate using anti-14-3-3b/a antibody at 1/500 dilution.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-14-3-3b/a antibody. Counter stained with hematoxylin.| Positive Control | mouse liver Hela |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100 IHC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
