Application
Fig1: Western blot analysis of Neuron-specific class III b-tubulin on Hela using anti-Neuron-specific class III b-tubulin antibody at 1/1,000 dilution.Application
Fig2: ICC staining Neuron-specific class III b-tubulin (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Neuron-specific class III b-tubulin (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of MCF-7 cells with Neuron-specific class III b-tubulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | Neuron-specific class III β-tubulin [A8-D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | beta 3 tubulin antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody |
| Product description | Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors. |
| Immunogen | Synthetic peptide (KLH-coupled) within human/mouse 1-50 aa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:500-1:1,000 IHC:1:100-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | beta 3 tubulin antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm. Cytoskeleton. Microtubule. |
Application
Fig1: Western blot analysis of Neuron-specific class III b-tubulin on Hela using anti-Neuron-specific class III b-tubulin antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Neuron-specific class III b-tubulin (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Neuron-specific class III b-tubulin (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of MCF-7 cells with Neuron-specific class III b-tubulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | Hela, MCF-7, SH-SY-5Y, rat brain tissue, human colon caner tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:500-1:1,000 IHC:1:100-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
