Application
Fig1: Western blot analysis on recombinant protein with His tag using anti-6| Product Name | 6X His tag [8-F0] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 His epitope tag antibody Hexa His tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody Polyhistidine Tag antibody |
| Product description | A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6 |
| Immunogen | synthetic peptide (KLH-coupled) |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Concentration | 2mg/ml |
| Alternative Names | 6 His epitope tag antibody Hexa His tag antibody HHHHHH epitope tag antibody HHHHHH tag antibody His tag antibody Polyhistidine Tag antibody |
|---|---|
| Molecular Weight(MW) | ～60 KDa |
Application
Fig1: Western blot analysis on recombinant protein with His tag using anti-6| Positive Control | Recombinant protein with 6 |
|---|---|
| Application Notes | WB:1:250~1:1000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | -20℃. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. |
