The fundamental structure of IgG contains two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus tetrameric quaternary structure. Light chain, which is usually same in a natural IgG molecule, includes κ chain and λ chain, but κ or λ antibody can exist in one body simultaneously. A heavy chain includes Fab- and Fc-Fragments. This antibody can recognize rabbit IgG Fc fragments of heavy chains.
Immunogen
Rabbit IgG
Conjugation
HRP
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
WBICC/IF
WB:1:5,000 ELISA:1:5,000
Species Reactivity
Rabbit
Concentration
2mg/ml
Target Information
Molecular Weight(MW)
50 kDa
Cellular Localization
Serum plasma
Application
Application
Fig1: M1003-7 was used at dilution 1/5000 (lane 1), 1/10000 (lane 2) and 1/50000 (lane 3) with rabbit IgG in WB.
All lanes proteins at 10 ng per lane.