Application
Fig1: Western blot analysis of FAM3A on recombinant protein using anti-FAM3A antibody at 1/1000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of A431 cells with FAM3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | FAM3A [7-B9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2-19 antibody 2.19 antibody Cytokine-like protein 2-19 antibody DLD antibody DXS560S antibody FAM3A antibody FAM3A_HUMAN antibody family with sequence similarity 3, member A antibody Protein FAM3A antibody XAP 7 antibody |
| Product description | The FAM3 gene family is a novel cytokine-like gene family, which includes four members designated as FAM3A, FAM3B, FAM3C, and FAM3D. FAM3A is a secreted protein belonging to the FAM3 family of four alpha -helix bundle cytokines. It is expressed in multiple tissues. Mature human FAM3A shares approximately 93% amino acid sequence identity with the mouse and rat protein.FAM3A belongs to a novel cytokine-like gene family, and its physiological role remains largely unknown. FAM3A activated Akt through the p110α catalytic subunit of PI3K in an insulin-independent manner. Blockade of P2 ATP receptors or downstream phospholipase C (PLC) and IP3R and removal of medium calcium all significantly reduced FAM3A-induced increase in cytosolic free Ca2+ levels and attenuated FAM3A-mediated PI3K/Akt activation. Moreover, FAM3A-induced Akt activation was completely abolished by the inhibition of calmodulin (CaM). |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHCFC |
| WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | 2-19 antibody
2.19 antibody
Cytokine-like protein 2-19 antibody
DLD antibody
DXS560S antibody
FAM3A antibody
FAM3A_HUMAN antibody
family with sequence similarity 3 member A antibody Protein FAM3A antibody XAP 7 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of FAM3A on recombinant protein using anti-FAM3A antibody at 1/1000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-FAM3A antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of A431 cells with FAM3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | FAM3A recombinant protein, human kidney tissue, human fetal skeletal muscle tissue, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 FC:1:100~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
