Application
Fig1: Western blot analysis of V5-tag on recombinant protein using anti-V5-tag antibody at 1/10,000 dilution.| Product Name | V5-tag [A10-D0-A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GKPIPNPLLGLDST epitope tag antibody GKPIPNPLLGLDST tag antibody Protein Rev antibody Regulator of expression of viral proteins antibody rev antibody V5 epitope tag antibody |
| Product description | The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). V5 tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Invitrogen V5 tag antibodies are designed to reliably detect recombinant V5-tagged proteins. |
| Immunogen | Peptide GKPIPNPLLGLDST (V5 epitope) conjugated with KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC/IF |
| WB:1:10,000 ELISA:1:20,000-1:50,000 |
|
| Concentration | 2mg/ml |
| Alternative Names | GKPIPNPLLGLDST epitope tag antibody GKPIPNPLLGLDST tag antibody Protein Rev antibody Regulator of expression of viral proteins antibody rev antibody V5 epitope tag antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
Application
Fig1: Western blot analysis of V5-tag on recombinant protein using anti-V5-tag antibody at 1/10,000 dilution.| Positive Control | Recombinant protein. |
|---|---|
| Application Notes | WB:1:10,000 ELISA:1:20,000-1:50,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
