Application
Fig1: Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.Application
Fig2: Western blot analysis of Beta-actin on different cell lysates using anti- Beta-actin antibody at 1/10000 dilution. Positive?control: Lane 1: NIH/3T3 Lane 2: PC12 Lane 3: MCF-7 Lane 4: HepG2 Lane 5: Hela Lane 6: Mouse lungApplication
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.| Product Name | Beta-actin [A2-F6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody |
| Product description | Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:10,000 ICC:1:100-1:200 IHC:1:100-1:200 FC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | A26C1A antibody
A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin cytoplasmic 1 N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody |
|---|---|
| Molecular Weight(MW) | 43kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P60709 |
|---|
Application
Fig1: Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.
Application
Fig2: Western blot analysis of Beta-actin on different cell lysates using anti- Beta-actin antibody at 1/10000 dilution. Positive?control: Lane 1: NIH/3T3 Lane 2: PC12 Lane 3: MCF-7 Lane 4: HepG2 Lane 5: Hela Lane 6: Mouse lung
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.| Positive Control | NIH/3T3, PC12, MCF-7, HepG2, Hela, human colon |
|---|---|
| Application Notes | WB:1:10,000 ICC:1:100-1:200 IHC:1:100-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 ℃. Stable for 12 months from date of receipt. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.23% Sodium Azide. |
