WB
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody at 1/1,000 dilution. Lane 1: TF-1 cell lysate, Lane 2: Jurkat cell lysate (negative), Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Species: Human, Site: Spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/200.| Product Name | CD11b Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. |
| Immunogen | Recombinant protein within human CD11b 500-750 aa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | FCIF-PIHCWB |
| WB:1:1000 IHC:1:1000 IF-P:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Alternative Names | antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
Complement receptor type 3 alpha subunit antibody CR 3 alpha chain (CR3A) antibody CR 3 alpha chain antibody CR-3 alpha chain antibody CR3 antibody CR3A antibody F730045J24Rik antibody Integrin Alpha M antibody Integrin alpha M chain antibody Integrin alpha-M antibody Integrin beta 2 alpha subunit antibody Integrin subunit alpha M antibody integrin alpha M (complement component 3 receptor 3 subunit) antibody ITAM_HUMAN antibody ITGAM antibody Leukocyte adhesion receptor MO1 antibody Ly-40 antibody MAC 1 antibody Mac-1a antibody MAC1 antibody Mac1 alpha subunit antibody MAC1A antibody Macrophage antigen alpha polypeptide antibody MGC117044 antibody Mo1 alpha subunit antibody MO1A antibody Neutrophil adherence receptor alpha M subunit antibody Neutrophil adherence receptor antibody SLEB6 antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa(Observed band size: 170 kDa) |
| Cellular Localization | Membrane |
| SwissProt ID | P11215 |
|---|
WB
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody at 1/1,000 dilution. Lane 1: TF-1 cell lysate, Lane 2: Jurkat cell lysate (negative), Lysates/proteins at 10 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Species: Human, Site: Spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/200.| Application Notes | WB:1:1000 IHC:1:1000 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
