Application
Fig1: Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.Application
Fig2: ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Product Name | TNNT2 [5-C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cardiac muscle troponin T antibody Cardiomyopathy dilated 1D (autosomal dominant) antibody Cardiomyopathy hypertrophic 2 antibody CMD1D antibody CMH2 antibody CMPD2 antibody cTnT antibody LVNC6 antibody MGC3889 antibody OTTHUMP00000033864 antibody OTTHUMP00000033865 antibody OTTHUMP00000033866 antibody OTTHUMP00000033867 antibody OTTHUMP00000033870 antibody OTTHUMP00000218095 antibody RCM3 antibody TNNT 2 antibody TNNT2 antibody TNNT2_HUMAN antibody TnTc antibody Troponin T cardiac muscle antibody Troponin T type 2 (cardiac) antibody Troponin T type 2 cardiac antibody Troponin T, cardiac muscle antibody Troponin T2 cardiac antibody |
| Product description | Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).Troponin T type 2 (cTnT,TNNT2) is a cardiac troponin T isoform expressed in the human heart, that is essential for calcium-regulated myofibrillar ATPase activity. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury. |
| Immunogen | Synthetic peptide within human TNNT2 50-100 aa. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:2,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Cardiac muscle troponin T antibody
Cardiomyopathy dilated 1D (autosomal dominant) antibody
Cardiomyopathy hypertrophic 2 antibody
CMD1D antibody
CMH2 antibody
CMPD2 antibody
cTnT antibody
LVNC6 antibody
MGC3889 antibody
OTTHUMP00000033864 antibody
OTTHUMP00000033865 antibody
OTTHUMP00000033866 antibody
OTTHUMP00000033867 antibody
OTTHUMP00000033870 antibody
OTTHUMP00000218095 antibody
RCM3 antibody
TNNT 2 antibody
TNNT2 antibody
TNNT2_HUMAN antibody
TnTc antibody
Troponin T cardiac muscle antibody
Troponin T type 2 (cardiac) antibody
Troponin T type 2 cardiac antibody
Troponin T cardiac muscle antibody Troponin T2 cardiac antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa |
| Cellular Localization | Cytoskeleton. Cytosol. |
Application
Fig1: Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.
Application
Fig2: ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.| Positive Control | Human heart tissue lysate, SK-Br-3, mouse heart tissue. |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
