ICC/IF
ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).ICC/IF
ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).IHC
Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using anti- DNMT3L mouse monoclonal antibody.| Product Name | DNMT3L Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | DNA (cytosine-5)-methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. |
| Immunogen | Recombinant protein within human DNMT3L aa 1-250/386. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200-1:500 IHC:1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Purification | Protein G |
| Alternative Names | Cytosine 5 methyltransferase 3 like protein antibody DNA (cytosine 5 ) methyltransferase 3 like antibody DNA (cytosine-5)-methyltransferase 3-like antibody DNA cytosine 5 methyltransferase 3 like protein antibody DNA methyltransferase 3 like protein antibody DNM3L_HUMAN antibody Dnmt 3L antibody Dnmt3l antibody Human cytosine 5 methyltransferase 3 like protein antibody MGC1090 antibody |
|---|---|
| Molecular Weight(MW) | 44kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9UJW3 |
|---|
ICC/IF
ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
ICC/IF
ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
IHC
Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using anti- DNMT3L mouse monoclonal antibody.| Application Notes | ICC/IF:1:200-1:500 IHC:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
