Application
Fig1: Western blot analysis on different cell lysates using anti-HSP27 mouse mAb.| Product Name | HSP27 [10-8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock 27kDa protein antibody 28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 28 antibody |
| Product description | Hsp27 is a chaperone of the sHsp(small heat shock protein) group among ubiquitin, α-crystallin,Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and?cell differentiation. They also take part in signal transduction. The main function of Hsp27 is to provide thermotolerance in vivo, cytoprotection, and support of cell survival under stress conditions. More specialized functions of Hsp27 are manifold and complex. In vitro it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. Hsp27 is also involved in the apoptotic signalling pathway. Hsp27 interacts with the outer mitochondrial membranes and interferes with the activation of?cytochrome c/Apaf-1/dATP complex and therefore inhibits the activation of procaspase-9. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:1,000-1:2,000 ICC:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Heat shock 27kDa protein antibody
28 kDa heat shock protein antibody CMT2F antibody DKFZp586P1322 antibody epididymis secretory protein Li 102 antibody Estrogen regulated 24 kDa protein antibody Estrogen-regulated 24 kDa protein antibody Heat shock 25kDa protein 1 antibody Heat shock 27 kDa protein antibody Heat shock 27kD protein 1 antibody Heat shock 27kDa protein 1 antibody Heat shock 28kDa protein 1 antibody Heat Shock Protein 27 antibody Heat shock protein beta 1 antibody Heat shock protein beta-1 antibody heat shock protein family B (small) member 1 antibody HEL-S-102 antibody HMN2B antibody HS.76067 antibody Hsp 25 antibody HSP 27 antibody Hsp 28 antibody Hsp B1 antibody Hsp25 antibody HSP27 antibody Hsp28 antibody HspB1 antibody HSPB1_HUMAN antibody SRP27 antibody Stress responsive protein 27 antibody Stress-responsive protein 28 antibody |
|---|---|
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cytoplasm, nucleus |
| SwissProt ID | P04792 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-HSP27 mouse mAb.| Positive Control | HepG2, Hela, Jurkat, A431 |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
