Application
Fig1: Western blot analysis on NIH/3T3 cell lysates using anti- Glut4 mouse mAb.Application
Fig2: ICC staining Glut4 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | Glucose Transporter GLUT4 [3-A10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4 |
| Product description | Under conditions of low insulin, most GLUT4 is sequestered in intracellular vesicles in muscle and fat cells. Insulin induces a rapid increase in the uptake of glucose by inducing the translocation of GLUT4 from these vesicles to the plasma membrane. As the vesicles fuse with the plasma membrane, GLUT4 transporters are inserted and become available for transporting glucose, and glucose absorption increases. Muscle contraction stimulates muscle cells to translocate GLUT4 receptors to their surfaces. This is especially true in cardiac muscle, where continuous contraction can be relied upon; but is observed to a lesser extent in skeletal muscle. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:2,000-1:5,000 ICC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Glucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2 facilitated glucose transporter member 4 |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cell membrane |
| SwissProt ID | P14672 |
|---|
Application
Fig1: Western blot analysis on NIH/3T3 cell lysates using anti- Glut4 mouse mAb.
Application
Fig2: ICC staining Glut4 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | NIH/3T3 |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
