Application
Fig1: Western blot analysis on cell lysates using anti- GRP78 mouse mAb.Application
Fig2: ICC staining GRP78 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GRP78 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | GRP78 BiP [C9-9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
| Product description | Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| WB:1:2,000-1:5,000 ICC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
|---|---|
| Molecular Weight(MW) | 78kDa |
| Cellular Localization | Cytoplasm, endoplasmic reticulum lumen |
| SwissProt ID | P11021 |
|---|
Application
Fig1: Western blot analysis on cell lysates using anti- GRP78 mouse mAb.
Application
Fig2: ICC staining GRP78 in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GRP78 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | HepG2, mouse liver, MCF-7 |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
