Application
Fig1: ICC staining SIRT1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- SIRT1 mouse mAb.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti- SIRT1 mouse mAb.| Product Name | SIRT1 [7-C5-B2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 75SirT1 antibody hSIR2 antibody hSIRT1 antibody HST2, S. cerevisiae, homolog of antibody NAD dependent deacetylase sirtuin 1 antibody NAD dependent protein deacetylase sirtuin 1 antibody OTTHUMP00000198111 antibody OTTHUMP00000198112 antibody Regulatory protein SIR2 homolog 1 antibody SIR1_HUMAN antibody SIR2 antibody SIR2 like 1 antibody SIR2 like protein 1 antibody SIR2, S.cerevisiae, homolog-like 1 antibody SIR2-like protein 1 antibody SIR2ALPHA antibody SIR2L1 antibody Sirt1 antibody SirtT1 75 kDa fragment antibody Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody Sirtuin 1 antibody Sirtuin type 1 antibody |
| Product description | SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARγ, and the PPARγ coactivator-1α (PGC-1α) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBICCIHC |
| IHC:1:200 ICC:1:200-1:500 WB:1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | 75SirT1 antibody
hSIR2 antibody
hSIRT1 antibody
HST2 S. cerevisiae homolog of antibody NAD dependent deacetylase sirtuin 1 antibody NAD dependent protein deacetylase sirtuin 1 antibody OTTHUMP00000198111 antibody OTTHUMP00000198112 antibody Regulatory protein SIR2 homolog 1 antibody SIR1_HUMAN antibody SIR2 antibody SIR2 like 1 antibody SIR2 like protein 1 antibody SIR2 S.cerevisiae homolog-like 1 antibody SIR2-like protein 1 antibody SIR2ALPHA antibody SIR2L1 antibody Sirt1 antibody SirtT1 75 kDa fragment antibody Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody Sirtuin 1 antibody Sirtuin type 1 antibody |
|---|---|
| Molecular Weight(MW) | 110kDa |
| Cellular Localization | Nucleus, Cytoplasm, |
| SwissProt ID | Q96EB6 |
|---|
Application
Fig1: ICC staining SIRT1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- SIRT1 mouse mAb.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti- SIRT1 mouse mAb.| Positive Control | Hela, human lung carcinoma tissue, human colon carcinoma tissue |
|---|---|
| Application Notes | IHC:1:200 ICC:1:200-1:500 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
