Application
Fig1: Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysateApplication
Fig2: ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig3: ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).Application
Fig4: Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | ICAM-1 [J0-F7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen identified by monoclonal antibody BB2 antibody BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody ICAM1 antibody ICAM1_HUMAN antibody intercellular adhesion molecule 1 (CD54), human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells, BB2 antibody |
| Product description | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. |
| Immunogen | Recombinant protein within human ICAM-1 aa 30-300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2c |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000: ICC:1:50-1:100: FC:1:50-1:100: |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Antigen identified by monoclonal antibody
BB2 antibody
BB 2 antibody BB2 antibody CD 54 antibody CD_antigen=CD54 antibody CD54 antibody Cell surface glycoprotein P3.58 antibody Human rhinovirus receptor antibody ICAM 1 antibody ICAM-1 antibody ICAM1 antibody ICAM1_HUMAN antibody intercellular adhesion molecule 1 (CD54) human rhinovirus receptor antibody Intercellular adhesion molecule 1 antibody Major group rhinovirus receptor antibody MALA 2 antibody MALA2 antibody MyD 10 antibody MyD10 antibody P3.58 antibody Surface antigen of activated B cells BB2 antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P05362 |
|---|
Application
Fig1: Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate
Application
Fig2: ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig3: ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Application
Fig4: Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Positive Control | Raji cell lysate, Daudi cell lysate, Hela, HUVEC, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000: ICC:1:50-1:100: FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
