Application
Fig1: Western blot analysis on different cell lysates using anti-PTP1B Mouse mAb. Positive control: Lane 1: A431 Lane 2: Hela Lane 3: HepG2 Lane 4: A549Application
Fig2： Immunocytochemical staining of Hela cells using anti-PTP1B Mouse mAb.Application
Fig3： Immunocytochemical staining of A549 cells using anti-PTP1B Mouse mAb.Application
Fig4： Immunocytochemical staining of HepG2 cells using anti-PTP1B Mouse mAb.Application
Fig5： Flow cytometric analysis of Jurkat cells with PTP1B antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Product Name | PTP1B [D0-C7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PTP1B antibody Non receptor tyrosine phosphatase 1 antibody Protein phosphotyrosylphosphatase 1B antibody Protein tyrosine phosphatase 1B antibody Protein tyrosine phosphatase non receptor type 1 antibody Protein tyrosine phosphatase placental antibody Protein-tyrosine phosphatase 1B antibody PTN1_HUMAN antibody PTP 1B antibody PTP-1B antibody PTPN 1 antibody PTPN1 antibody Tyrosine protein phosphatase non receptor type 1 antibody Tyrosine-protein phosphatase non-receptor type 1 antibody |
| Product description | The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | PTP1B antibody Non receptor tyrosine phosphatase 1 antibody Protein phosphotyrosylphosphatase 1B antibody Protein tyrosine phosphatase 1B antibody Protein tyrosine phosphatase non receptor type 1 antibody Protein tyrosine phosphatase placental antibody Protein-tyrosine phosphatase 1B antibody PTN1_HUMAN antibody PTP 1B antibody PTP-1B antibody PTPN 1 antibody PTPN1 antibody Tyrosine protein phosphatase non receptor type 1 antibody Tyrosine-protein phosphatase non-receptor type 1 antibody |
|---|---|
| Molecular Weight(MW) | 50kDa |
| Cellular Localization | Endoplasmic reticulum membrane |
| SwissProt ID | P18031 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-PTP1B Mouse mAb. Positive control: Lane 1: A431 Lane 2: Hela Lane 3: HepG2 Lane 4: A549
Application
Fig2： Immunocytochemical staining of Hela cells using anti-PTP1B Mouse mAb.
Application
Fig3： Immunocytochemical staining of A549 cells using anti-PTP1B Mouse mAb.
Application
Fig4： Immunocytochemical staining of HepG2 cells using anti-PTP1B Mouse mAb.
Application
Fig5： Flow cytometric analysis of Jurkat cells with PTP1B antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Positive Control | Hela, A431, A549, HepG2 |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
