Application
Fig1: Western blot analysis of CD31 on THP-1 cells lysates using anti-CD31 antibody at 1/1,000 dilution.Application
Fig2: ICC staining CD31 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CD31 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse uterus muscle tissue using anti-CD31 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Product Name | CD31/PECAM-1 [7-A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adhesion molecule antibody CD31 antibody CD31 antigen antibody CD31 EndoCAM antibody EndoCAM antibody FLJ34100 antibody FLJ58394 antibody GPIIA antibody GPIIA' antibody PECA1 antibody PECA1_HUMAN antibody Pecam 1 antibody PECAM 1 CD31 EndoCAM antibody PECAM antibody PECAM-1 antibody Pecam1 antibody Platelet and endothelial cell adhesion molecule 1 antibody Platelet endothelial cell adhesion molecule antibody Platelet/endothelial cell adhesion molecule 1 antibody |
| Product description | Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 (ICAM-1), also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily. PECAM-1 (platelet/endothelial cell adhesion molecule-1), also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, platelets and a subpopulation of T cells. VCAM-1 (vascular cell adhesion molecule-1) was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor (TNF) and lipopolysaccharide (LPS). The KALIG gene encodes a nerve cell adhesion molecule (NCAM)-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism. |
| Immunogen | Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICC/IFIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:1,000-1:5,000 FC:1:50-1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 2mg/ml |
| Alternative Names | Adhesion molecule antibody
CD31 antibody CD31 antigen antibody CD31 EndoCAM antibody EndoCAM antibody FLJ34100 antibody FLJ58394 antibody GPIIA antibody GPIIA' antibody PECA1 antibody PECA1_HUMAN antibody Pecam 1 antibody PECAM 1 CD31 EndoCAM antibody PECAM antibody PECAM-1 antibody Pecam1 antibody Platelet and endothelial cell adhesion molecule 1 antibody Platelet endothelial cell adhesion molecule antibody Platelet/endothelial cell adhesion molecule 1 antibody |
|---|---|
| Molecular Weight(MW) | 82kDa(Observed: 130 kDa) |
| Cellular Localization | Cell junction. Cell membrane. Membrane. |
Application
Fig1: Western blot analysis of CD31 on THP-1 cells lysates using anti-CD31 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining CD31 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CD31 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse uterus muscle tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Positive Control | THP-1, HUVEC, PMVEC, NIH/3T3, Jurkat, MCF-7, human tonsil tissue, human kidney tissue, human uterus tissue, mouse kidney tissue, mouse uterus tissue |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:1,000-1:5,000 FC:1:50-1:100: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
