Application
Fig1: Western blot analysis on different cell lysates using anti-Hsp90 alpha Mouse mAb. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: PC12 Lane 4: NIH/3T3Application
Fig2： Immunocytochemical staining of Hela cells using anti-Hsp90 alpha Mouse mAb.Application
Fig3： Immunocytochemical staining of SW480 cells using anti-Hsp90 alpha Mouse mAb.Application
Fig4： Immunocytochemical staining of Ags cells using anti-Hsp90 alpha Mouse mAb.Application
Fig5： Immunocytochemical staining of NIH/3T3 cells using anti-Hsp90 alpha Mouse mAb.Application
Fig6： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Hsp90 alpha Mouse mAb.Application
Fig7： Immunohistochemical analysis of paraffin- embedded human gastric carcinoma tissue using anti-Hsp90 alpha Mouse mAb.Application
Fig8： Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-Hsp90 alpha Mouse mAb.Application
Fig9： Flow cytometric analysis of Hela cells with Hsp90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Product Name | Hsp90 alpha [A10-A5-D2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EL52 antibody epididymis luminal secretory protein 52 antibody Heat shock 86 kDa antibody heat shock 90kD protein 1, alpha antibody Heat shock 90kD protein 1, alpha like 4 antibody heat shock 90kD protein, alpha-like 4 antibody Heat shock 90kDa protein 1 alpha antibody Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody Heat shock protein HSP 90-alpha antibody HS90A_HUMAN antibody HSP 86 antibody HSP86 antibody Hsp89 antibody HSP89A antibody Hsp90 antibody HSP90A antibody HSP90AA1 antibody HSP90ALPHA antibody HSP90N antibody HSPC1 antibody HSPCA antibody HSPCAL1 antibody HSPCAL4 antibody HSPN antibody LAP 2 antibody LAP2 antibody lipopolysaccharide-associated protein 2 antibody LPS-associated protein 2 antibody Renal carcinoma antigen NY-REN-38 antibody |
| Product description | The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | EL52 antibody
epididymis luminal secretory protein 52 antibody
Heat shock 86 kDa antibody
heat shock 90kD protein 1 alpha antibody Heat shock 90kD protein 1 alpha like 4 antibody heat shock 90kD protein alpha-like 4 antibody Heat shock 90kDa protein 1 alpha antibody Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody Heat shock protein HSP 90-alpha antibody HS90A_HUMAN antibody HSP 86 antibody HSP86 antibody Hsp89 antibody HSP89A antibody Hsp90 antibody HSP90A antibody HSP90AA1 antibody HSP90ALPHA antibody HSP90N antibody HSPC1 antibody HSPCA antibody HSPCAL1 antibody HSPCAL4 antibody HSPN antibody LAP 2 antibody LAP2 antibody lipopolysaccharide-associated protein 2 antibody LPS-associated protein 2 antibody Renal carcinoma antigen NY-REN-38 antibody |
|---|---|
| Molecular Weight(MW) | 90kDa |
| Cellular Localization | Cytoplasm, Melanosome, Cell membrane |
| SwissProt ID | P07900 |
|---|
Application
Fig1: Western blot analysis on different cell lysates using anti-Hsp90 alpha Mouse mAb. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: PC12 Lane 4: NIH/3T3
Application
Fig2： Immunocytochemical staining of Hela cells using anti-Hsp90 alpha Mouse mAb.
Application
Fig3： Immunocytochemical staining of SW480 cells using anti-Hsp90 alpha Mouse mAb.
Application
Fig4： Immunocytochemical staining of Ags cells using anti-Hsp90 alpha Mouse mAb.
Application
Fig5： Immunocytochemical staining of NIH/3T3 cells using anti-Hsp90 alpha Mouse mAb.
Application
Fig6： Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Hsp90 alpha Mouse mAb.
Application
Fig7： Immunohistochemical analysis of paraffin- embedded human gastric carcinoma tissue using anti-Hsp90 alpha Mouse mAb.
Application
Fig8： Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-Hsp90 alpha Mouse mAb.
Application
Fig9： Flow cytometric analysis of Hela cells with Hsp90 alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.| Positive Control | Hela, PC12, NIH/3T3, SW480, Ags, human kidney tissue, human gastric carcinoma tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
