Application
Fig1: Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.Application
Fig2: ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Product Name | PP2A(alpha+beta) [A0-G7] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PP2A A antibody PP2A alpha antibody PP2A B antibody PP2A beta antibody PP2A-alpha antibody PP2AA_HUMAN antibody PP2Ac antibody PP2CB antibody PPP2CA antibody PPP2CB antibody Protein phosphatase 2 catalytic subunit alpha isoform antibody Protein phosphatase 2 catalytic subunit beta isoform antibody Replication protein C antibody RP C antibody RP-C antibody Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody |
| Product description | PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICC |
| WB:1:2,000-1:5,000 ICC:1:100-1:500 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | PP2A A antibody PP2A alpha antibody PP2A B antibody PP2A beta antibody PP2A-alpha antibody PP2AA_HUMAN antibody PP2Ac antibody PP2CB antibody PPP2CA antibody PPP2CB antibody Protein phosphatase 2 catalytic subunit alpha isoform antibody Protein phosphatase 2 catalytic subunit beta isoform antibody Replication protein C antibody RP C antibody RP-C antibody Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.
Application
Fig2: ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.| Positive Control | A431 cell lysate, Hela, MCF-7, SHG-44. |
|---|---|
| Application Notes | WB:1:2,000-1:5,000 ICC:1:100-1:500 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
