Application
Fig1: Western blot analysis of HSPA5 on different cells lysates using anti-HSPA5 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: HepG2 Line3: NIH/3T3Application
Fig2: ICC staining HSPA5 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining HSPA5 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining HSPA5 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.Application
Fig9: Flow cytometric analysis of Hela cells with HSPA5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody| Product Name | HSPA5 [A5-C9-G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
| Product description | The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity. |
| Immunogen | Peptides |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:1000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Endoplasmic reticulum lumen. Melanosome. Cytoplasm. |
| SwissProt ID | P11021 |
|---|
Application
Fig1: Western blot analysis of HSPA5 on different cells lysates using anti-HSPA5 antibody at 1/1,000 dilution. Positive control： Line1: Hela Line2: HepG2 Line3: NIH/3T3
Application
Fig2: ICC staining HSPA5 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining HSPA5 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining HSPA5 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Application
Fig9: Flow cytometric analysis of Hela cells with HSPA5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody| Positive Control | HepG2, Hela, 293T, NIH/3T3, human liver tissue, human hepatocellular carcinoma tissue, human colon cancer tissue, human breast carcinoma tissue. |
|---|---|
| Application Notes | WB:1:1000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -391℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
