Application
Fig1: Western blot analysis of PTBP1 on different cell lysate using anti-PTBP1 antibody at 1/1,000 dilution. Positive control：Lane1: DaudiLane2: HelaLane3: PC-12Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.| Product Name | PTBP1 [5C1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 57 kDa RNA binding protein PPTB 1 antibody 57 kDa RNA-binding protein PPTB-1 antibody Heterogeneous nuclear ribonucleoprotein I antibody Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody hnRNP I antibody HNRNP-I antibody HNRNPI antibody HNRPI antibody MGC10830 antibody MGC8461 antibody Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody Polypyrimidine Tract Binding Protein 1 antibody Polypyrimidine tract binding protein antibody Polypyrimidine tract-binding protein 1 antibody pPTB antibody PTB 1 antibody PTB 2 antibody PTB 3 antibody PTB 4 antibody PTB antibody PTB T antibody PTB1 antibody PTB2 antibody PTB3 antibody PTB4 antibody PTBP 1 antibody PTBP1 antibody PTBP1_HUMAN antibody PTBT antibody RNA Binding Protein antibody |
| Product description | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. hnRNP I, also designated polypyrimidine tract-binding protein (PTB), and its homolog hnRNP L bind to the 3' end of introns to modulate alternative splicing mechanisms of pre-mRNAs in normal cells and the translation of several viruses, including hepatitis C virus (HCV). The human hnRNP I gene maps to chromosome 19p13.3 and encodes a protein that is localized in the nucleoplasm. hnRNP L, like hnRNP I, is also localized in the nucleoplasm. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanRat |
| Concentration | 2mg/ml |
| Alternative Names | 57 kDa RNA binding protein PPTB 1 antibody 57 kDa RNA-binding protein PPTB-1 antibody Heterogeneous nuclear ribonucleoprotein I antibody Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody hnRNP I antibody HNRNP-I antibody HNRNPI antibody HNRPI antibody MGC10830 antibody MGC8461 antibody Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody Polypyrimidine Tract Binding Protein 1 antibody Polypyrimidine tract binding protein antibody Polypyrimidine tract-binding protein 1 antibody pPTB antibody PTB 1 antibody PTB 2 antibody PTB 3 antibody PTB 4 antibody PTB antibody PTB T antibody PTB1 antibody PTB2 antibody PTB3 antibody PTB4 antibody PTBP 1 antibody PTBP1 antibody PTBP1_HUMAN antibody PTBT antibody RNA Binding Protein antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PTBP1 on different cell lysate using anti-PTBP1 antibody at 1/1,000 dilution. Positive control：Lane1: DaudiLane2: HelaLane3: PC-12
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.| Positive Control | Daudi, Hela, PC-12, human tonsil tissue, human colon cancer tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 IHC:1:20~1:100 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
