Application
Fig1: Western blot analysis of Akt2 expression in MCF7 whole cell lysate.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining (A).Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing granular cytoplasmic staining of Islets of Langerhans and glandular cells (B).| Product Name | Akt2 [2A1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Akt2 antibody AKT2_HUMAN antibody HIHGHH antibody murine thymoma viral (v-akt) homolog-2 antibody PKB antibody PKB beta antibody PKBB antibody PKBBETA antibody PRKBB antibody Protein kinase Akt 2 antibody Protein kinase Akt-2 antibody Protein kinase B beta antibody rac protein kinase beta antibody RAC-BETA antibody RAC-beta serine/threonine-protein kinase antibody RAC-PK-beta antibody v akt murine thymoma viral oncogene homolog 2 antibody |
| Product description | The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation that is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Akt proteins become phosphorylated and activated in Insulin/IGF-I-stimulated cells by an upstream kinase, and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Akt2 antibody AKT2_HUMAN antibody HIHGHH antibody murine thymoma viral (v-akt) homolog-2 antibody PKB antibody PKB beta antibody PKBB antibody PKBBETA antibody PRKBB antibody Protein kinase Akt 2 antibody Protein kinase Akt-2 antibody Protein kinase B beta antibody rac protein kinase beta antibody RAC-BETA antibody RAC-beta serine/threonine-protein kinase antibody RAC-PK-beta antibody v akt murine thymoma viral oncogene homolog 2 antibody |
|---|---|
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane |
| SwissProt ID | P31751 |
|---|
Application
Fig1: Western blot analysis of Akt2 expression in MCF7 whole cell lysate.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining (A).Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing granular cytoplasmic staining of Islets of Langerhans and glandular cells (B).| Positive Control | Hela, MCF7, Langerhans, glandular, human pancreas tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
