Application
Fig1: Western Blot analysis of Ang-2 expression in HUV-EC-C (A) and TF-1 (B) whole cell lysates.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification.| Product Name | Angiopoietin-2 [4A2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGPT 2 antibody Agpt2 antibody ANG 2 antibody ANG-2 antibody ANG2 antibody Angiopoietin 2a antibody Angiopoietin 2B antibody Angiopoietin-2 antibody Angiopoietin2 antibody ANGP2_HUMAN antibody ANGPT 2 antibody Angpt2 antibody Tie2 ligand antibody |
| Product description | Tie-1 and Tie-2 (also designated Tek) are novel cell surface receptor tyrosine kinases. The extracellular domain of Tie-1 has an unusual multidomain structure consisting of a cluster of three epidermal growth factor homology motifs localized between two immunoglobulin-like loops, which are followed by three Fibronectin type III repeats next to the transmembrane region. Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential Fibrinogen-like domain at the carboxy-terminus and coiled-coil regions in the amino-terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | AGPT 2 antibody Agpt2 antibody ANG 2 antibody ANG-2 antibody ANG2 antibody Angiopoietin 2a antibody Angiopoietin 2B antibody Angiopoietin-2 antibody Angiopoietin2 antibody ANGP2_HUMAN antibody ANGPT 2 antibody Angpt2 antibody Tie2 ligand antibody |
|---|---|
| Molecular Weight(MW) | 62-70kDa |
| Cellular Localization | Secreted |
| SwissProt ID | O15123 |
|---|
Application
Fig1: Western Blot analysis of Ang-2 expression in HUV-EC-C (A) and TF-1 (B) whole cell lysates.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification.| Positive Control | HUV-EC-C, TF-1, human placenta tissue |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
