Application
Fig1: Western blot analysis of citrate synthase expression in non-transfected 293T (A), mouse citrate synthase transfected 293T (B) and K-562 (C) whole cell lysates.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes (B).| Product Name | Citrate synthase [1G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CISY_HUMAN antibody Citrate synthase antibody Citrate synthase, mitochondrial antibody citrate synthetase antibody Cs antibody EC 2.3.3 antibody EC 2.3.3.1 antibody |
| Product description | Citrate synthase (CS) is a 466 amino acid mitochondrial matrix protein that functions as the first and rate-limiting enzyme of the tricarboxylic acid cycle. Essential in mitochondrial respiration and involved in the conversion of glucose to lipid, citrate synthase is found the great majority of cells that are capable of oxidative metabolism. The gene encoding citrate synthase maps to human chromosome 12q13.3, which is transcribed into two alternatively spliced variants designated CSa and CSb. Human chromosome 12 encodes over 1,100 genes, comprises approximately 4.5% of the human genome and is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders. |
| Immunogen | An epitope mapping between amino acids 259-292 of citrate synthase of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CISY_HUMAN antibody
Citrate synthase antibody
Citrate synthase mitochondrial antibody citrate synthetase antibody Cs antibody EC 2.3.3 antibody EC 2.3.3.1 antibody |
|---|---|
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Mitochondrion matrix |
| SwissProt ID | O75390 |
|---|
Application
Fig1: Western blot analysis of citrate synthase expression in non-transfected 293T (A), mouse citrate synthase transfected 293T (B) and K-562 (C) whole cell lysates.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes (B).| Positive Control | Hela, 293T, K-562, human heart muscle tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
