Application
Fig1: Western blot analysis of claudin-1 expression in non-transfected 293T (A), human claudin-1 transfected 293T (B) and SCC-4 (C) whole cell lysates.Application
Fig2： Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed,paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells (B). .| Product Name | Claudin 1 [8G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Claudin-1 antibody Claudin1 antibody CLD 1 antibody CLD1 antibody CLD1_HUMAN antibody CLDN 1 antibody Cldn1 antibody ILVASC antibody SEMP 1 antibody SEMP1 antibody Senescence associated epithelial membrane protein 1 antibody Senescence associated epithelial membrane protein antibody Senescence-associated epithelial membrane protein antibody |
| Product description | The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-1 is a multi-pass membrane protein that is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Defects in the gene encoding claudin-1 are the cause of ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH), an autosomal recessive syndrome characterized by vulgar type ichthyosis, scalp hypotrichosis, scarring alopecia and sclerosing cholangitis. |
| Immunogen | Amino acids 168-207 at the C-terminus of claudin-1 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
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| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | Claudin-1 antibody Claudin1 antibody CLD 1 antibody CLD1 antibody CLD1_HUMAN antibody CLDN 1 antibody Cldn1 antibody ILVASC antibody SEMP 1 antibody SEMP1 antibody Senescence associated epithelial membrane protein 1 antibody Senescence associated epithelial membrane protein antibody Senescence-associated epithelial membrane protein antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cell junction, tight junction. Cell membrane. |
| SwissProt ID | O95832 |
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Application
Fig1: Western blot analysis of claudin-1 expression in non-transfected 293T (A), human claudin-1 transfected 293T (B) and SCC-4 (C) whole cell lysates.
Application
Fig2： Immunofluorescence staining of formalin-fixed Hep G2 cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed,paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells (B). .| Positive Control | Human claudin-1 transfected 293T, SCC-4, human bone marrow tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
