Application
Fig1: Westernblot analysis of c-Myc expressionin untreated (A) and (E)-4-Hydroxyhexenal treated (B) Jurkat whole cell lysates. Note down-regulation of c-Myc in lane B.Application
Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining (A). Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining (B).| Product Name | c-Myc [6G6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AU016757 antibody Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc-related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Myelocytomatosis oncogene antibody Niard antibody Nird antibody Oncogene Myc antibody OTTHUMP00000158589 antibody Proto-oncogene c-Myc antibody Protooncogene homologous to myelocytomatosis virus antibody RNCMYC antibody Transcription factor p64 antibody Transcriptional regulator Myc-A antibody V-Myc avian myelocytomatosis viral oncogene homolog antibody v-myc myelocytomatosis viral oncogene homolog (avian) antibody |
| Product description | C-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-PFCM |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 FCM |
|
| Species Reactivity | HumanMouseRatMonkey |
| Concentration | 2mg/ml |
| Alternative Names | AU016757 antibody
Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc-related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Myelocytomatosis oncogene antibody Niard antibody Nird antibody Oncogene Myc antibody OTTHUMP00000158589 antibody Proto-oncogene c-Myc antibody Protooncogene homologous to myelocytomatosis virus antibody RNCMYC antibody Transcription factor p64 antibody Transcriptional regulator Myc-A antibody V-Myc avian myelocytomatosis viral oncogene homolog antibody v-myc myelocytomatosis viral oncogene homolog (avian) antibody |
|---|---|
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P01106 |
|---|
Application
Fig1: Westernblot analysis of c-Myc expressionin untreated (A) and (E)-4-Hydroxyhexenal treated (B) Jurkat whole cell lysates. Note down-regulation of c-Myc in lane B.
Application
Fig2： Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining (A). Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining (B).| Positive Control | Jurkat, COS |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 FCM |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
