Application
Fig1: A.Western blot analysis of Epac expression in Caki (A) and CHO (B) whole celllysates.Application
Fig2： B.Western blot analysis of Epac expression in HeLa whole cell lysate.| Product Name | Epac1 [4G3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | bcm910 antibody CAMP GEFI antibody cAMP regulated guanine nucleotide exchange factor I antibody CAMPGEFI antibody CGEF 1 antibody CGEF1 antibody EPA1 antibody Epac 1 antibody EPAC antibody EPAC1 antibody Exchange factor directly activated by cAMP 1 antibody Exchange protein directly activated by cAMP 1 antibody MGC21410 antibody RAP guanine nucleotide exchange factor antibody Rap guanine nucleotide exchange factor (GEF) 3 antibody RAP guanine nucleotide exchange factor 3 antibody Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody RAPGEF3 antibody |
| Product description | 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanRatHamster |
| Concentration | 2mg/ml |
| Alternative Names | bcm910 antibody CAMP GEFI antibody cAMP regulated guanine nucleotide exchange factor I antibody CAMPGEFI antibody CGEF 1 antibody CGEF1 antibody EPA1 antibody Epac 1 antibody EPAC antibody EPAC1 antibody Exchange factor directly activated by cAMP 1 antibody Exchange protein directly activated by cAMP 1 antibody MGC21410 antibody RAP guanine nucleotide exchange factor antibody Rap guanine nucleotide exchange factor (GEF) 3 antibody RAP guanine nucleotide exchange factor 3 antibody Rap1 guanine nucleotide exchange factor directly activated by cAMP antibody RAPGEF3 antibody |
|---|---|
| Molecular Weight(MW) | 99 kDa |
| Cellular Localization | Endomembrane system |
| SwissProt ID | O95398 |
|---|
Application
Fig1: A.Western blot analysis of Epac expression in Caki (A) and CHO (B) whole celllysates.
Application
Fig2： B.Western blot analysis of Epac expression in HeLa whole cell lysate.| Positive Control | Hela, Caki, CHO. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
