Application
Fig1: A.Western blot analysis of Fibronectin expression in 3611-RF (A) and NIH/3T3 (B) whole cell lysates.Application
Fig2： B.Western blot analysis of Fibronectin expression in HepG2 (A), CCD-1064Sk (B) whole cell lysates and human platelet extract (C).| Product Name | Fibronectin [3G4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIG antibody Cold insoluble globulin antibody Cold-insoluble globulin antibody DKFZp686F10164 antibody DKFZp686H0342 antibody DKFZp686I1370 antibody DKFZp686O13149 antibody ED B antibody Fibronectin 1 antibody FINC antibody FINC_HUMAN antibody FN antibody FN1 antibody FNZ antibody GFND antibody GFND2 antibody LETS antibody Migration stimulating factor antibody MSF antibody Ugl-Y3 antibody |
| Product description | Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren’s disease. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CIG antibody Cold insoluble globulin antibody Cold-insoluble globulin antibody DKFZp686F10164 antibody DKFZp686H0342 antibody DKFZp686I1370 antibody DKFZp686O13149 antibody ED B antibody Fibronectin 1 antibody FINC antibody FINC_HUMAN antibody FN antibody FN1 antibody FNZ antibody GFND antibody GFND2 antibody LETS antibody Migration stimulating factor antibody MSF antibody Ugl-Y3 antibody |
|---|---|
| Molecular Weight(MW) | 220kDa |
| Cellular Localization | Secreted |
| SwissProt ID | P02751 |
|---|
Application
Fig1: A.Western blot analysis of Fibronectin expression in 3611-RF (A) and NIH/3T3 (B) whole cell lysates.
Application
Fig2： B.Western blot analysis of Fibronectin expression in HepG2 (A), CCD-1064Sk (B) whole cell lysates and human platelet extract (C).| Positive Control | 3611-RF, NIH/3T3, HepG2, CCD-1064Sk. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
