Application
Fig1: Western blot analysis of Hint1 expression in Jurkat (A) and HepG2 (B) whole cell lysates.Application
Fig2： Immunofluorescencestaining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | Hint1 [4G12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine 5' monophosphoramidase antibody Adenosine 5''-monophosphoramidase antibody HINT 1 antibody HINT1 antibody HINT1_HUMAN antibody Histidine triad nucleotide binding protein 1 antibody Histidine triad nucleotide-binding protein 1 antibody PKCI 1 antibody PKCI-1 antibody PKCI1 antibody PRKCNH1 antibody Protein kinase C inhibitor 1 antibody Protein kinase C interacting protein 1 antibody Protein kinase C-interacting protein 1 antibody |
| Product description | Hint1 (histidine triad nucleotide binding protein 1), also known as PRKCNH1 or PKCI1, is a 126 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one HIT (histidine triad) domain. Widely expressed and existing as a homodimer, Hint1 functions to hydrolyze adenosine 5'-monophosphoramidate substrates, such as AMP-N-alanine methyl ester, AMP-morpholidate and AMP-α-acetyl lysine methyl ester, and may also inhibit PKC function and influence apoptosis. Knockdown of Hint1 mRNA results in downregulation of p53 and Bax, suggesting that Hint1 is a haploinsufficient tumor suppressor. Also, it is suspected that promoter hypermethylation of the gene encoding Hint1 may play a role in hepatocarcinogenesis. |
| Immunogen | Amino acids 8-47 mapping near the N-terminus of Hint 1 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate). IF:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | Adenosine 5' monophosphoramidase antibody Adenosine 5''-monophosphoramidase antibody HINT 1 antibody HINT1 antibody HINT1_HUMAN antibody Histidine triad nucleotide binding protein 1 antibody Histidine triad nucleotide-binding protein 1 antibody PKCI 1 antibody PKCI-1 antibody PKCI1 antibody PRKCNH1 antibody Protein kinase C inhibitor 1 antibody Protein kinase C interacting protein 1 antibody Protein kinase C-interacting protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P49773 |
|---|
Application
Fig1: Western blot analysis of Hint1 expression in Jurkat (A) and HepG2 (B) whole cell lysates.
Application
Fig2： Immunofluorescencestaining of methanol-fixed HeLa cells showing cytoplasmic localization.| Positive Control | Hela, Jurkat, HepG2. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein (1 ml of cell lysate). IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
