Application
Fig1: Western blot analysis of hnRNP A1 expression in Jurkat (A) and HeLa (B) nuclear extracts.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of glandular cells.| Product Name | hnRNP A1 [1G2] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNRNPA 1 antibody Helix destabilizing protein antibody Helix-destabilizing protein antibody Heterogeneous nuclear ribonucleoprotein A1 antibody Heterogeneous nuclear ribonucleoprotein A1B protein antibody Heterogeneous nuclear ribonucleoprotein B2 protein antibody Heterogeneous nuclear ribonucleoprotein core protein A1 antibody hnRNP A1 antibody hnRNP core protein A1 antibody HNRNPA1 antibody HNRPA1 antibody MGC102835 antibody Nuclear ribonucleoprotein particle A1 protein antibody ROA1_HUMAN antibody Single strand DNA binding protein UP1 antibody Single strand RNA binding protein antibody Single-strand RNA-binding protein antibody |
| Product description | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | HNRNPA 1 antibody Helix destabilizing protein antibody Helix-destabilizing protein antibody Heterogeneous nuclear ribonucleoprotein A1 antibody Heterogeneous nuclear ribonucleoprotein A1B protein antibody Heterogeneous nuclear ribonucleoprotein B2 protein antibody Heterogeneous nuclear ribonucleoprotein core protein A1 antibody hnRNP A1 antibody hnRNP core protein A1 antibody HNRNPA1 antibody HNRPA1 antibody MGC102835 antibody Nuclear ribonucleoprotein particle A1 protein antibody ROA1_HUMAN antibody Single strand DNA binding protein UP1 antibody Single strand RNA binding protein antibody Single-strand RNA-binding protein antibody |
|---|---|
| Molecular Weight(MW) | 29/34/39kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P09651 |
|---|
Application
Fig1: Western blot analysis of hnRNP A1 expression in Jurkat (A) and HeLa (B) nuclear extracts.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing nuclear staining of glandular cells.| Positive Control | Hela, Jurkat, human nasopharynx tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-500 IHC:1:50-500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
