Application
Fig1: Western blot analysis of Iba1 on human PBL whole cell lysate using anti-Iba1 antibody at 1/1,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat C6 glioblastoma tissue using anti-Iba1 antibody. Counter stained with hematoxylin.| Product Name | Iba1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody G1 antibody G1 putative splice variant of allograft inflamatory factor 1 antibody IBA 1 antibody IBA1 antibody interferon gamma responsive transcript antibody Interferon responsive transcript 1 antibody interferon responsive transcript factor 1 antibody Ionized calcium binding adapter molecule 1 antibody Ionized calcium-binding adapter molecule 1 antibody ionized calcium-binding adapter molecule antibody IRT 1 antibody IRT1 antibody Microglia response factor antibody MRF1 antibody Protein g1 antibody |
| Product description | Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC). |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFCIP |
| WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 IP:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | AIF 1 antibody AIF-1 antibody Aif1 antibody AIF1 protein antibody AIF1_HUMAN antibody Allograft inflammatory factor 1 antibody Allograft inflammatory factor 1 splice variant G antibody allograft inflammatory factor-1 splice variant Hara-1 antibody balloon angioplasty responsive transcription antibody BART 1 antibody G1 antibody G1 putative splice variant of allograft inflamatory factor 1 antibody IBA 1 antibody IBA1 antibody interferon gamma responsive transcript antibody Interferon responsive transcript 1 antibody interferon responsive transcript factor 1 antibody Ionized calcium binding adapter molecule 1 antibody Ionized calcium-binding adapter molecule 1 antibody ionized calcium-binding adapter molecule antibody IRT 1 antibody IRT1 antibody Microglia response factor antibody MRF1 antibody Protein g1 antibody |
|---|---|
| Molecular Weight(MW) | 17kDa |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| SwissProt ID | P55008 |
|---|
Application
Fig1: Western blot analysis of Iba1 on human PBL whole cell lysate using anti-Iba1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat C6 glioblastoma tissue using anti-Iba1 antibody. Counter stained with hematoxylin.| Positive Control | Human PBL whole cell lysate, rat brain, rat C6 glioblastoma tissue. |
|---|---|
| Application Notes | WB:1:250~1:1000 ICC:1:100~1:200 IHC:1:20~1:100 FC:1:100~1:200 IP:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
