Application
Fig1: Western blot analysis of IRP-2 expression in Jurkat whole cell lysate.Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.| Product Name | IREB2 [1G3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACO3 antibody FLJ23381 antibody IRE BP2 antibody IRE-BP 2 antibody IREB 2 antibody IREB2 antibody IREB2_HUMAN antibody Iron regulatory protein 2 antibody iron responsive element binding protein 2 antibody Iron-responsive element-binding protein 2 antibody IRP 2 antibody IRP2 antibody IRP2AD antibody OTTHUMP00000185022 antibody |
| Product description | Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins: IRP-1 and -2 (iron regulatory protein-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE-BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. |
| Immunogen | Full-length recombinant IRP-2 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | ACO3 antibody FLJ23381 antibody IRE BP2 antibody IRE-BP 2 antibody IREB 2 antibody IREB2 antibody IREB2_HUMAN antibody Iron regulatory protein 2 antibody iron responsive element binding protein 2 antibody Iron-responsive element-binding protein 2 antibody IRP 2 antibody IRP2 antibody IRP2AD antibody OTTHUMP00000185022 antibody |
|---|---|
| Molecular Weight(MW) | 105kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P48200 |
|---|
Application
Fig1: Western blot analysis of IRP-2 expression in Jurkat whole cell lysate.
Application
Fig2： Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.| Positive Control | Jurkat, human adrenal gland tissue. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
