Application
Fig1: Western blot analysis of Flk-1 expression in non-transfected (A) and mouse Flk-1 transfected (B) 293T whole cell lysates.Application
Fig2： Western blot analysis of Flk-1 expression in VEGF-treated HUV-EC-C whole cell lysate.| Product Name | KDR / VEGFR-2 [1G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD309 antibody CD309 antigen antibody EC 2.7.10.1 antibody Fetal liver kinase 1 antibody FLK-1 antibody FLK1 antibody FLK1, mouse, homolog of antibody Kdr antibody Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody Kinase insert domain receptor antibody KRD1 antibody Ly73 antibody Protein tyrosine kinase receptor FLK1 antibody Protein-tyrosine kinase receptor flk-1 antibody soluble VEGFR2 antibody Tyrosine kinase growth factor receptor antibody Vascular endothelial growth factor receptor 2 antibody VEGFR 2 antibody VEGFR antibody VEGFR-2 antibody VEGFR2 antibody VGFR2_HUMAN antibody |
| Product description | Three cell membrane receptor tyrosine kinases, Flt (also designated VEGF-R1), Flk-1 (also designated VEGF-R2) and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulinlike sequences in their extracellular domain. These receptors exhibit high degrees of sequence relatedness to each other as well as lesser degrees of relatedness to the class III receptors including CSF-1/Fms, PDGR, SLFR/Kit and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand. |
| Immunogen | Amino acids 1158-1345 mapping at the C-terminus of the Flk-1 of mouse origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | CD309 antibody
CD309 antigen antibody
EC 2.7.10.1 antibody
Fetal liver kinase 1 antibody
FLK-1 antibody
FLK1 antibody
FLK1 mouse homolog of antibody Kdr antibody Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody Kinase insert domain receptor antibody KRD1 antibody Ly73 antibody Protein tyrosine kinase receptor FLK1 antibody Protein-tyrosine kinase receptor flk-1 antibody soluble VEGFR2 antibody Tyrosine kinase growth factor receptor antibody Vascular endothelial growth factor receptor 2 antibody VEGFR 2 antibody VEGFR antibody VEGFR-2 antibody VEGFR2 antibody VGFR2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 200kDa |
| Cellular Localization | Cytoplasm, Nucleus, Secreted, Cell junction Endoplasmic reticulum, Cell membrane, Cytoplasmic vesicle, Early endosome |
| SwissProt ID | P35968 |
|---|
Application
Fig1: Western blot analysis of Flk-1 expression in non-transfected (A) and mouse Flk-1 transfected (B) 293T whole cell lysates.
Application
Fig2： Western blot analysis of Flk-1 expression in VEGF-treated HUV-EC-C whole cell lysate.| Positive Control | 293T, HUV-EC-C |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
