Application
Fig1: Western blot analysis of Tak1 expression in non-transfected (A) and mouse Tak1 transfected (B) 293T whole cell lysates.Application
Fig2： Western blot analysis of Tak1 expression in non-transfected 293 (A), human Tak1 transfected 293 (B) and 3611-RF (C) whole cell lysates.| Product Name | MAP3K7 [1G5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | M3K7_HUMAN antibody MAP3K 7 antibody Map3k7 antibody MEKK7 antibody Mitogen activated protein kinase kinase kinase 7 antibody Mitogen-activated protein kinase kinase kinase 7 antibody TAK1 antibody TGF beta activated kinase 1 antibody TGF-beta-activated kinase 1 antibody TGF1a antibody Transforming growth factor beta activated kinase 1 antibody Transforming growth factor-beta-activated kinase 1 antibody |
| Product description | Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine/ threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase sup-pressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation. |
| Immunogen | Amino acids 1-579 representing full length Tak1 (TGFβ-activated kinase) of mouse origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIF |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | M3K7_HUMAN antibody MAP3K 7 antibody Map3k7 antibody MEKK7 antibody Mitogen activated protein kinase kinase kinase 7 antibody Mitogen-activated protein kinase kinase kinase 7 antibody TAK1 antibody TGF beta activated kinase 1 antibody TGF-beta-activated kinase 1 antibody TGF1a antibody Transforming growth factor beta activated kinase 1 antibody Transforming growth factor-beta-activated kinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytoplasm, Cell membrane |
| SwissProt ID | O43318 |
|---|
Application
Fig1: Western blot analysis of Tak1 expression in non-transfected (A) and mouse Tak1 transfected (B) 293T whole cell lysates.
Application
Fig2： Western blot analysis of Tak1 expression in non-transfected 293 (A), human Tak1 transfected 293 (B) and 3611-RF (C) whole cell lysates.| Positive Control | 293T, 293, 3611-RF. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
