Application
Fig1: Western blot analysis of Na+/K+-ATPase α expression in human kidney (A) and human brain (B) tissue extracts.Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing membrane staining of glandular cells (B).| Product Name | Na+/K+ ATPase [1G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATPase Na+/K+ transporting alpha antibody adenosinetriphosphatase antibody AT1A1_HUMAN antibody ATP1A1 antibody ATP1A4 antibody ATP1AL2 antibody ATP1B antibody ATP1B1 antibody ATPase Na+/K+ transporting alpha 1 polypeptide antibody ATPase Na+/K+ transporting alpha 4 polypeptide antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody ATPase, Na+/K+ transporting, beta 1 polypeptide antibody Beta 1-subunit of Na(+),K(+)-ATPase antibody Na(+)/K(+) ATPase alpha-1 subunit antibody Na(+)/K(+) ATPase alpha-4 subunit antibody Na+, K+ ATPase alpha subunit antibody Na+/K+ ATPase 1 antibody Na+/K+ ATPase 4 antibody Na+/K+ ATPase, alpha-D polypeptide antibody Na, K-ATPase beta-1 polypeptide antibody Na, K-ATPase, alpha-A catalytic polypeptide antibody Na,K-ATPase catalytic subunit alpha-A protein antibody Na,K-ATPase subunit alpha-C antibody polypeptide-like 2 antibody Sodium pump 1 antibody sodium pump 4 antibody Sodium pump subunit alpha-1 antibody sodium pump subunit alpha-4 antibody sodium pump subunit beta-1 antibody sodium-potassium ATPase catalytic subunit alpha-1 antibody sodium-potassium ATPase catalytic subunit alpha-4 antibody sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody sodium-potassium ATPase, alpha 4 polypeptide antibody sodium-potassium-ATPase, alpha 1 polypeptide antibody Sodium/potassium transporting ATPase alpha 1 chain antibody Sodium/potassium transporting ATPase subunit beta 1 antibody sodium/potassium-dependent ATPase beta-1 subunit antibody sodium/potassium-transporting ATPase alpha-4 chain antibody sodium/potassium-transporting ATPase beta-1 chain antibody Sodium/potassium-transporting ATPase subunit alpha-1 antibody sodium/potassium-transporting ATPase subunit alpha-4 antibody |
| Product description | The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase. |
| Immunogen | Amino acids 551-850 of Na+/K+-ATPase α1 of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIPIFIHC-P |
| WB:1:2,000-1:1,0000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ATPase Na+/K+ transporting alpha antibody
adenosinetriphosphatase antibody
AT1A1_HUMAN antibody
ATP1A1 antibody
ATP1A4 antibody
ATP1AL2 antibody
ATP1B antibody
ATP1B1 antibody
ATPase Na+/K+ transporting alpha 1 polypeptide antibody
ATPase Na+/K+ transporting alpha 4 polypeptide antibody
ATPase Na+/K+ transporting beta 1 polypeptide antibody
ATPase Na+/K+ transporting alpha polypeptide-like 2 antibody ATPase Na+/K+ transporting beta 1 polypeptide antibody Beta 1-subunit of Na(+) K(+)-ATPase antibody Na(+)/K(+) ATPase alpha-1 subunit antibody Na(+)/K(+) ATPase alpha-4 subunit antibody Na+ K+ ATPase alpha subunit antibody Na+/K+ ATPase 1 antibody Na+/K+ ATPase 4 antibody Na+/K+ ATPase alpha-D polypeptide antibody Na K-ATPase beta-1 polypeptide antibody Na K-ATPase alpha-A catalytic polypeptide antibody Na K-ATPase catalytic subunit alpha-A protein antibody Na K-ATPase subunit alpha-C antibody polypeptide-like 2 antibody Sodium pump 1 antibody sodium pump 4 antibody Sodium pump subunit alpha-1 antibody sodium pump subunit alpha-4 antibody sodium pump subunit beta-1 antibody sodium-potassium ATPase catalytic subunit alpha-1 antibody sodium-potassium ATPase catalytic subunit alpha-4 antibody sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody sodium-potassium ATPase alpha 4 polypeptide antibody sodium-potassium-ATPase alpha 1 polypeptide antibody Sodium/potassium transporting ATPase alpha 1 chain antibody Sodium/potassium transporting ATPase subunit beta 1 antibody sodium/potassium-dependent ATPase beta-1 subunit antibody sodium/potassium-transporting ATPase alpha-4 chain antibody sodium/potassium-transporting ATPase beta-1 chain antibody Sodium/potassium-transporting ATPase subunit alpha-1 antibody sodium/potassium-transporting ATPase subunit alpha-4 antibody |
|---|---|
| Molecular Weight(MW) | 100-143kDa |
| Cellular Localization | Melanosome, Cell membrane |
| SwissProt ID | P05023 |
|---|
Application
Fig1: Western blot analysis of Na+/K+-ATPase α expression in human kidney (A) and human brain (B) tissue extracts.
Application
Fig2： Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing membrane staining of glandular cells (B).| Positive Control | Hela, human duodenum tissue, human kidney tissue, human brain tissue. |
|---|---|
| Application Notes | WB:1:2,000-1:1,0000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF:1:50-1:500 IHC:1:50-1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
