Application
Fig1: Western blot analysis of TBK1 expression in RAW264.7 (A), MCF7 (B) and KNRK (C) whole cell lysates.| Product Name | NAK / TBK1 [2G1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 2.7.11.1 antibody FLJ11330 antibody NAK antibody NF kappa B activating kinase antibody NF kB activating kinase antibody NF-kappa-B-activating kinase antibody Serine/threonine protein kinase TBK 1 antibody Serine/threonine protein kinase TBK1 antibody Serine/threonine-protein kinase TBK1 antibody T2K antibody TANK binding kinase 1 antibody TANK-binding kinase 1 antibody TBK 1 antibody Tbk1 antibody TBK1_HUMAN antibody |
| Product description | The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IκB-α and specifically phosphorylates IκB-α on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues. TBK1 was shown to complex with TRAF2 and TANK in the NFκB activation pathway. TBK1 shares homology with IKKα and IKKβ in the amino-terminal half, which includes the kinase domain. |
| Immunogen | peptide |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBIP |
| WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | EC 2.7.11.1 antibody FLJ11330 antibody NAK antibody NF kappa B activating kinase antibody NF kB activating kinase antibody NF-kappa-B-activating kinase antibody Serine/threonine protein kinase TBK 1 antibody Serine/threonine protein kinase TBK1 antibody Serine/threonine-protein kinase TBK1 antibody T2K antibody TANK binding kinase 1 antibody TANK-binding kinase 1 antibody TBK 1 antibody Tbk1 antibody TBK1_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 80 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | Q9UHD2 |
|---|
Application
Fig1: Western blot analysis of TBK1 expression in RAW264.7 (A), MCF7 (B) and KNRK (C) whole cell lysates.| Positive Control | RAW264.7, MCF7, KNRK. |
|---|---|
| Application Notes | WB:1:100-1:1,000 IP:1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
